Composite

Part:BBa_K3149000

Designed by: Triana Mayra S�nchez Huerta   Group: iGEM19_TecMonterrey_GDL   (2019-10-16)


TRZ1 receptor under the control of the inducible pBAD (strong) promoter

We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay.

T--TecMonterrey_GDL--Results-7.png

10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 503
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None