Composite

Part:BBa_K3146012:Design

Designed by: Milva Khandakar   Group: iGEM19_Westminster_UK   (2019-10-18)


RhlA with promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 130
    Illegal BamHI site found at 690
    Illegal XhoI site found at 866
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355
    Illegal BsaI.rc site found at 539


Design Notes

Composite part which has RhlA coding region (BBa_K3146007)


Source

RBS used for better expression of RhIA

References