Coding

Part:BBa_K3142004:Design

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-10-13)


11S globulin seed storage protein（A11Sg）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for lactic acid bacteria.

Source

Amaranthus hypochondriacus

References

[1] Barba De La Rosa AP, Herrera-Estrella A, Utsumi S, et al. Molecular characterization, cloning and structural analysis of a cDNA encoding an amaranth globulin. J Plant Physiol 1996;149(5):527–32.

[2] Chen S, Paredes O. Isolation and characterization of the 11S globulin from amaranth seeds. J Food Biochem 1997;21(6):53–65.