Composite
Part:BBa_K3142002:Design
Designed by: Luo Xiaolin Group: iGEM19_SZPT-CHINA (2019-09-28)
Acid-induced promoter and repressor lacR of lac operon
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal NgoMIV site found at 149
Illegal NgoMIV site found at 1052 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 989
Illegal SapI.rc site found at 718
Design Notes
It only applies to lactic acid bacteria
Source
Lactobacillus delbrueckii subsp. Lactis NCC936