Composite
Part:BBa_K3136014:Design
Designed by: Shiyuan Li Group: iGEM19_Shanghai_HS_United (2019-10-11)
J23100-B0034-GFP-ter-typeIIS-RBS-A
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 57
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 57 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 57
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 57
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the sacr between BioBrick RFC_10 and typeIIS assembly are different and may effect the protein expression, we constructed the four RBS to measure the expression strength.
Source
Artifial synthesis.