Coding

Part:BBa_K3134009:Design

Designed by: Shiyuan Li   Group: iGEM19_Nanjing_high-school   (2019-10-16)


Cas1-Cas2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We expected Cas1Cas2 to express when we were doing the first round light-sensitive experiments, but the results turned out that there was no increase in strip. Then we decided to see under which condition Cas1Cas2 was most likely to express. We designed an experiment in which we added different amount of IPTG and L-Arabinose to the system. At last we found out the most suitable condition and finished the light-sensitive experiments.


Source

PCR from E. coli MG1655

References