![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K313036:Design
Designed by: Tomoka Ao, Ryosuke Kamei, Ryo Kariyazono, Masahiko Shimizu, Kosuke Uekusa, Mami Nakatake Group: iGEM10_UT-Tokyo (2010-10-26)
MS2 phage cDNA (loading sequence and replicase-deficient)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal BamHI site found at 2057
Illegal XhoI site found at 1048
Illegal XhoI site found at 3214 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 1733
Illegal NgoMIV site found at 2437
Illegal NgoMIV site found at 3309 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1665
Illegal BsaI.rc site found at 163
Illegal BsaI.rc site found at 2257
Design Notes
We removed XbaI site, loading sequence and replicase region.
Source
We bought MS2 virus as RNA and made cDNA by reverse transcription.