Coding
GGS Linker

Part:BBa_K3128010:Design

Designed by: Mélodie Dupré   Group: iGEM19_Grenoble-Alpes   (2019-09-18)


Flexible Glycine Glycine Serine (GGS) Linker (54 aa)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

In the iGEM Grenoble 2019 project, these linkers are used in order to gain a certain flexibility with the adenylate subparts fused to the OmpX proteins. Thus allowing the utilization of a bacterial two-hybrid system in the external membrane of the bacteria.


Design Notes

The length of the linker has been chosen to ensure huge flexibility to the inter-domain of fusion proteins.


Source

This part has been created by synthesis from IDT.

References