Reporter
PLac_RFP
Part:BBa_K3128009:Design
Designed by: Lucas PINERO Group: iGEM19_Grenoble-Alpes (2019-09-16)
RFP protein under PLac promoter (with two restriction sites around the reporter)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 229
Illegal BamHI site found at 913 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 789
Illegal AgeI site found at 901 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To exchange the RFP gene by another gene two restrictions sites were added on both ends of the RFP gene : 5'_BglII_mRFP_BamHI_3'
Source
Davidson Synth-Aces iGEM plate