Part:BBa_K3126007

TgMTP1t2

The gene can express the TgMTP1t2 protein that can transfer nickel ions into yeast vacuoles. This year we want to use it to improve the tolerance of Saccharomyces cerevisiae to nickel ions.

Usage and Biology

The ability of Thlaspi goesingense to hyperaccumulate Ni seems to be governed in part by enhanced accumulation of Ni within leaf vacuoles. These proteins contain all of the features of cation-efflux family members, and evidence indicates they are derived from a single genomic sequence (TgMTP1) that gives rise to an unspliced (TgMTP1t1) and a spliced (TgMTP1t2) transcript. Heterologous expression of these transcripts in yeast lacking the TgMTP1 orthologues COT1 and ZRC1 complements the metal sensitivity of these yeast strains, suggesting that TgMTP1s are able to transport metal ions into the yeast vacuole in a manner similar to COT1 and ZRC1. The unspliced and spliced TgMTP1 variants differ within a histidine-rich putative metal-binding domain, and these sequence differences are reflected as alterations in the metal specificities of these metal ion transporters. When expressed in yeast, TgMTP1t1 confers the highest level of tolerance to Cd, Co, and Zn, whereas TgMTP1t2 confers the highest tolerance to Ni. TgMTP1 transcripts are highly expressed in T. goesingense compared with orthologues in the nonaccumulators Arabidopsis thaliana, Thlaspi arvense, and Brassica juncea. We propose that the high-level expression of TgMTP1 in T. goesingense accounts for the enhanced ability of this hyperaccumulator to accumulate metal ions within shoot vacuoles.(Michael W. Persans, Ken Nieman, and David E. Salt.2001) To view its function please see Part:BBa_K3126020

Schematic

Reference

Functional activity and role of cation-efflux family members in Ni hyperaccumulation in Thlaspi goesingense Michael W. Persans, Ken Nieman, and David E. Salt PNAS August 14, 2001 98 (17) 9995-10000; https://doi.org/10.1073/pnas.171039798 Edited by Maarten J. Chrispeels, University of California at San Diego, La Jolla, CA, and approved June 21, 2001 (received for review January 24, 2001)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 40
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]