Other

Part:BBa_K3117024:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)


(Glycine)x4-Serine Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our flexible linker is composed of small, non-polar (e.g. Gly) and polar (e.g. Ser or Thr) amino acids. By adjusting the copy number, in this case 4 repeats of GGGGS, the length of this GS linker can be optimized to achieve appropriate separation of the proteins of interest [1]. 3 repeats ensure a high level of flexibility.


Source

This part comes from genomic DNA.

References

[1]: Chen, Xiaoying; Zaro, Jennica L.; Shen, Wei-Chiang (2013): Fusion protein linkers: property, design and functionality. In: Advanced drug delivery reviews 65