Tag

Part:BBa_K3117005:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)


6xHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We considered which tag can be used for the purification of our protein. Choosing the histidine-tag, we thought about how many repeats of histidine are useful for our project and chose the 6xHis tag.


Source

This part comes from genomic sequence.

References

1. Booth, William T.; Schlachter, Caleb R.; Pote, Swanandi; Ussin, Nikita; Mank, Nicholas J.; Klapper, Vincent et al. (2018): Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability.