![](https://parts.igem.org/images/partbypart/icon_tag.png)
Tag
Part:BBa_K3117005:Design
Designed by: Lena Schorr Group: iGEM19_FAU_Erlangen (2019-10-13)
6xHis-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We considered which tag can be used for the purification of our protein. Choosing the histidine-tag, we thought about how many repeats of histidine are useful for our project and chose the 6xHis tag.
Source
This part comes from genomic sequence.
References
1. Booth, William T.; Schlachter, Caleb R.; Pote, Swanandi; Ussin, Nikita; Mank, Nicholas J.; Klapper, Vincent et al. (2018): Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability.