Project

Part:BBa_K3113320:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-13)


pCAG_mGag-HiBit-DHD154a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2491
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2421
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

L21S mutation to increased VLP assembly in mouse cells.


Source

CAG-Promotor, GAG: coat protein of HIV, HiBit: small part of nano luciferase, DHD154a: synthetic coiled-coil structure


References