Project

Part:BBa_K3113314:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-13)


pCAG_Avilabel-PSGL1-TMandCT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were performed to remove illegal restriction sites.


Source

CAG-Promotor, Avilabel: single-chain avidin, PSGL1: P-Selectin-Glycoprotein-Ligand-1,


References