Project

Part:BBa_K3113313:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-13)


pCAG_hArc-HiBit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2347
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were performed to remove illegal restriction sites.


Source

CAG-Promotor, hArc: Recombinant protein of human activity-regulated cytoskeleton-associated protein, HiBit: small part of nano luciferase


References