Coding

Part:BBa_K3113014

Designed by: Theresa Keil   Group: iGEM19_Munich   (2019-10-13)


NLuc

This sequence encodes a nano luciferase.

Usage

We used this part in the form of RNA to load into extracellular vesicles.


Biology

NLuc or NanoLuc is a small 19 kDA bioluminescence protein that is independent of ATP.[1]

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Boute, Nicolas et al. “NanoLuc Luciferase - A Multifunctional Tool for High Throughput Antibody Screening.” Frontiers in pharmacology vol. 7 27. 18 Feb. 2016, doi:10.3389/fphar.2016.00027
[edit]
Categories
Parameters
None