DNA

Part:BBa_K3112030

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-14)


Homologous arm in Saccharomyces cerevisiae V Chromosome 3

This is a segment about 2K from the centromere on the V chromosome of Saccharomyces cerevisiae, which is used to integrate the centromere of Yarrowia lipolytica with the tag into its genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 133
    Illegal PstI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None