Composite
TmEnc_iLOV

Part:BBa_K3111104:Design

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-30)


T. maritima encapsulin (6-His) fused to iLOV with a StrepII-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
    Illegal BglII site found at 586
    Illegal BamHI site found at 950
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 520
    Illegal SapI.rc site found at 551


Design Notes

-


Source

Encapsulin monomer is derived from Thermotoga maritima; iLOV is derived from Broad host range vector vector pCVD060.

References