Composite
TmEnc_iLOV
Part:BBa_K3111104:Design
Designed by: Matas Deveikis Group: iGEM19_UCL (2019-09-30)
T. maritima encapsulin (6-His) fused to iLOV with a StrepII-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 171
Illegal BglII site found at 586
Illegal BamHI site found at 950 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 520
Illegal SapI.rc site found at 551
Design Notes
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Source
Encapsulin monomer is derived from Thermotoga maritima; iLOV is derived from Broad host range vector vector pCVD060.