Coding

Part:BBa_K3110050:Design

Designed by: Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-16)


YebF-IL12-FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have put the YebF sequence in the N-terminus of IL12 so that IL12 also gets secreted out of E.coli along with YebF.

Source

We have synthesized the whole part from Twist Bioscience.

References