Part:BBa_K3110034

Medium Promoter Strong RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 328
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3650
  Illegal AgeI site found at 464
  Illegal AgeI site found at 927
  Illegal AgeI site found at 1365
  Illegal AgeI site found at 1524
  Illegal AgeI site found at 1536
  Illegal AgeI site found at 2282
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3087
  

Characterisation

The construct could not be characterised due to insufficient time.