Composite

Part:BBa_K3110031

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)


Strong Promoter Strong RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.

Characterisation

Due to insufficient time, we could not characterise this construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3650
    Illegal AgeI site found at 464
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1524
    Illegal AgeI site found at 1536
    Illegal AgeI site found at 2282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3087


[edit]
Categories
Parameters
None