Composite

Part:BBa_K3105672:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)


HRP-2A-AAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 1275
    Illegal BamHI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
    Illegal AgeI site found at 2422
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sequence optimised for Pichia pastoris

Source

HRP from Armoracia rusticana
AAO from Pleurotus eryngii

References