Reporter

Part:BBa_K3105670:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

eGFP was codon optimised for Pichia pastoris

Source

modified variant of natural GFP from Aequorea victoria (Zhang et al.)

References

Zhang, Guohong, et al. “An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells.” Biochemical and Biophysical Research Communications, 1996, doi:10.1006/bbrc.1996.1573.