Composite

Part:BBa_K3105666:Design

Designed by: Jinwen Yuan   Group: iGEM19_Uppsala_Universitet   (2019-08-26)


AsPink with ribosome standby site upstream of RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used a low copy number plasmid (pSB3K3) to show that we achieved a higher expression.


Source

Consists of AsPink (BBa_k1033906), including constitutive promoter (J23110) with RBS (B0034).

References