Composite

Part:BBa_K3102037

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-PFLB-Ter

This T7-RBS-PFLB-ter composite part is made of the pyruvate formate lyase enzyme which is essential in bacterial anaerobic metabolism and is responsible for converting pyruvate and coenzyme A (CoA) into formate and acetyl-CoA.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


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Categories
Parameters
None