Composite

Part:BBa_K3102036:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-MTRC-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 270


Design Notes

We mutated in the MtrC sequence with a Guanine into Cysteine and a Guanine into Adenine at the 1795bp and 1798bp position (G1795C & G1798A), respectively, because of the PstI enzyme (BBa_K3102020).

Source

This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1627

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Kouzuma A, Kasai T, Hirose A, Watanabe K. Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells. Front Microbiol. 2015;6(JUN):1–11.