Part:BBa_K3102031

T7-RBS-ACS-Ter

This T7-RBS-ACS-ter composite is made of the Acetyl-CoA Synthetase (BBa_K3102021) used to activate acetate to acetyl-CoA in an ATP-dependent manner.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 408
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 837
  
• 1000
  COMPATIBLE WITH RFC[1000]