Composite

Part:BBa_K3102023:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-CAE-ter (secreted CAE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 1234
    Illegal AgeI site found at 254
    Illegal AgeI site found at 341
    Illegal AgeI site found at 479
    Illegal AgeI site found at 662
    Illegal AgeI site found at 710
    Illegal AgeI site found at 878
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CAE:Sequence synthesized and optimized for E.coli on Geneius

Source

Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Moriyoshi K, Ohmoto T, Ohe T, Sakai K. Purification and characterization of an esterase involved in cellulose acetate degradation by neisseria sicca SB. Biosci Biotechnol Biochem. 1999;63(10):1708–13.

Moriyoshi K, Koma D, Yamanaka H, Ohmoto T, Sakai K. Functional analysis of the carbohydrate-binding module of an esterase from neisseria sicca SB involved in the degradation of cellulose acetate. Biosci Biotechnol Biochem. 2010;74(9):1940–2.