Coding

Part:BBa_K3098015:Design

Designed by: Jiongyi He   Group: iGEM19_WHU-China   (2019-10-04)


sfGFP+Avi-tag+His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Avi-tag can perform normal function whether added to C-terminal or N-terminal of the peptide.


Source

sfGFP is codon optimized from BBa_K2804006.

References