Composite
Part:BBa_K3096051:Design
Designed by: Jakob Keck, Patrick Mueller, Lina Widerspick, Famke Baeuerle Group: iGEM19_Tuebingen (2019-10-15)
CRISPR array that targets the genomic DNA of E.coli - expression controlled via the Mnt repressor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Trouble with the Synthesis of repeats. Target sequences had to lie in direct neighbourhood to a PAM sequence.
Source
Synthesis