Composite

Part:BBa_K3096035

Designed by: Famke Baeuerle, Patrick Mueller, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)

SynB1 with His-tag and GFP

Can be used for isolation (via His-tag) and visualization (via GFP) of the CPP SynB1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 806
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 676


References

[edit]
Categories
Parameters
None