Composite

Part:BBa_K3096012

Designed by: Famke Baeuerle, Patrick Mueller, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)

Glucose-dependent TetR expression

CAP promoter regulates expression in dependency of glucose. TetR binds to the pTet regulator inhibiting transcription. Anhydrotetracycline binds to TetR inhibiting its operation.

Usage and Biology

This part originates from the Lactose operon. The operator - binding site of LacI - was deleted to yield a lactose independent regulatory system. It is known that the Lactose operon also responds to catabolite repression, which is now the only regulatory element within the promoter, making it solely dependent on glucose. If the glucose concentration is LOW, the bacterial Adenylate Cyclase is active and synthesises cAMP (cyclic AMP), which interacts with CAP (catabolite activating protein). The CAP-cAMP complex then binds the CAP binding site potentially activating transcription of TetR. This consequently leads to the inactivation of the transcription from the pTet.

If the glucose concentration increases, the Catabolite Repression system will inhibit the expression of TetR and consequently the genes downstream of pTet could be transcribed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

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Categories
Parameters
None