Part:BBa_K3096008

NagC regulated Mnt repressor

Promoter induces expression in presence of GlcNAc6P. Mnt repressor under the control of the nagC promoter. Mnt is a repressor that can inhibit transcription when binding to its designated promoter.

Usage and Biology

Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen (Sicard et al. 2017). GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses its abilities to bind DNA, and therefore its respective regulation activity. In our case, nagC can consequently not serve as a repressor of the nag Operon nagBACDE, anymore (Barnhart et al. 2006, Knopka et al. 202). Thus, a repressor protein, the Mnt repressor of the Lambda phage, can be expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 195
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017); 7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
2. Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006); 188(14):5212–5219. doi:10.1128/JB.00234-06
3. Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012); 2012:489208. doi:10.6064/2012/489208