Part:BBa_K3096005

Constitutive FadR expression and regulated promoter

Const. FadR generator (BBa_K861052 contains promoter, RBS, FadR) - FadR promoter (BBa_K861060)- RBS (BBa_K3096000). FadR binds DNA and represses gene expression for beta-oxidation in the absence of long chain Acyl-CoA

Background

The intake of a fatty meal increases the fatty acid availability within the body and therefore their metabolite Acyl-CoA will increase. Long chain Acyl-CoA will bind constitutively expressed FadR and hence inhibits its activity (Feng et al. 2012). FadR is the repressor of the promoter pFad

Usage and Biology

The FadR expression system of E.Coli senses Acyl-CoA and activates fatty acid transport genes in vivo (Feng et al. 2012).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 902
  Illegal NheI site found at 925
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Feng Y, Cronan JE. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PLoS One. (2012); 7(9):e46275. doi:10.1371/journal.pone.0046275