DNA

Part:BBa_K3091000:Design

Designed by: YUHE SHEN   Group: iGEM19_SDSZ_China   (2019-10-16)


Polyphenol Oxidase [PPO]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1180
    Illegal PstI site found at 434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1180
    Illegal PstI site found at 434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1180
    Illegal PstI site found at 434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1180
    Illegal PstI site found at 434
    Illegal AgeI site found at 1009
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is blasted on NCBI and synthesized by Twist. When designed for Team SDSZ_China's project in2019, considering that the type of PPO chosen should catalyst a reaction that is not such important a threat to the integrity of cell membrane, the part with a sequence of PPO from potato was chosen.


Source

The sequence of the part is searched from NCBI and the sequence is synthesized to be inserted into a plasmid.

References