Composite

Part:BBa_K309032:Design

Designed by: Thai Phi   Group: iGEM10_Queens-Canada   (2010-10-27)


pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts PCRed out of genomic/plasmid DNA and ligated together using T4 ligase. A Kozak sequence was added in front of the reporter (eCFP) in order to facilitate the translation process.


Source

From C. elegans (pMec-7, unc-54 'UTR)and A. victoria (eCFP) DNA.

References