Composite
Part:BBa_K309032:Design
Designed by: Thai Phi Group: iGEM10_Queens-Canada (2010-10-27)
pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Parts PCRed out of genomic/plasmid DNA and ligated together using T4 ligase. A Kozak sequence was added in front of the reporter (eCFP) in order to facilitate the translation process.
Source
From C. elegans (pMec-7, unc-54 'UTR)and A. victoria (eCFP) DNA.