Regulatory

Part:BBa_K309011:Design

Designed by: Thai Phi   Group: iGEM10_Queens-Canada   (2010-10-22)


pHsp-3 (for use in C. elegans chassis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in front of the Hsp-3 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Hsp-3 gene.


Source

From C. elegans genomic DNA.

References