Composite
Part:BBa_K3089034:Design
Designed by: Aiai Dong Group: iGEM19_Greatbay_SCIE (2019-10-17)
T7 promoter+csgA-linker-mfp5-linker-sfGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal PstI site found at 400 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 400
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 585
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
Illegal PstI site found at 400 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal PstI site found at 400 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 780
Design Notes
Linkers are applied to prevent each component jamming together
Source
CsgA - E.coli MG1655 Mfp5 - Mytilus galloprovincialis