Composite
Part:BBa_K3089015:Design
Designed by: Aiai Dong Group: iGEM19_Greatbay_SCIE (2019-10-14)
fp1-linker-mgfp5-linker-fp1-His fusion protein for yeast expression
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 274
Illegal PstI site found at 23 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 274
Illegal PstI site found at 23 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 274
Illegal XhoI site found at 244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 274
Illegal PstI site found at 23 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 274
Illegal PstI site found at 23 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Redesign the sequence for it to fit yeast expression conditions 7*His affinity tag for purification of genome
Source
Hwang, D. S., Gim, Y., Yoo, H. J., & Cha, H. J. (2007). Practical recombinant hybrid mussel bioadhesive fp-151 fp-151 - mussel adhesive protein/foot protein type 1