![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K3086003:Design
Designed by: Nikila Ojili Group: iGEM19_Florida (2019-10-18)
msr +msd(rpoB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to insert the rpoB sequence into msd using ligation independent cloning. The msDNA (containing the rpoB target sequence) had to be designed such that the it would be homologous to the Okazaki fragment during DNA replication.
Source
PFF745 plasmid from lab of Prof. Tim Lu (iGEM registry Bba_K1681000)