Coding
Part:BBa_K3084000:Design
Designed by: Lucas Coppens Group: iGEM19_KU_LEUVEN (2019-10-15)
Venus Yellow Fluorescent Protein (YFP), S-TIP37 codon optimised
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 424
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our team looked more closely into the genome of S-TIP37 phage to be able to identify the capsid protein as a strongly expressed protein. We decided to take over the codon distribution found on the phage genome in our design.
Source
Part:BBa_K592101 from the iGEM registry