Part:BBa_K3081060:Design

pBAD-dCas9-T7-M+

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal NheI site found at 2321
  Illegal NheI site found at 6316
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal BamHI site found at 4600
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  Illegal SapI.rc site found at 5369
  

Design Notes

The module of inducible T7 RNAP expression can be integrated into the chromosome or put on another plasmid.

Source

This composite part is created using some basic parts from the registry.