Composite

Part:BBa_K3081059

Designed by: Xiaoying Qiao   Group: iGEM19_Peking   (2019-10-16)


dCas9 J23119 M+ with the thermo-sensitive transcription factor TCI42

This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(BBa_K3081060), including dCas9 and sgRNA. The sgRNA called M+ targets a 20bp DNA sequence in E.coli OriC, which will arrest and inhibit the genome replication initiation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 753
    Illegal NheI site found at 776
    Illegal NheI site found at 5286
    Illegal NheI site found at 5309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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