Composite
Part:BBa_K3081056:Design
Designed by: Xiaoying Qiao Group: iGEM19_Peking (2019-10-16)
sgRNA generator with promoter J23119
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 251
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 441
Illegal BsaI.rc site found at 37
Design Notes
no
Source
E.coli