Composite

Part:BBa_K3081046:Design

Designed by: Xiaoying Qiao   Group: iGEM19_Peking   (2019-10-19)


pBAD-dCas9-T7-sgRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 6708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
    Illegal XhoI site found at 6437
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6627
    Illegal BsaI.rc site found at 6223
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5369


Design Notes

no


Source

E.coli

References