Part:BBa_K3071011

Clp-binding site I from gumB operon (CBS I)

Description

This is the regulatory element from the gum operon of Xanthomonas campestris pv. camperstris. The sequence is centered in the nt -145.5 of gumB upstream sequence.

Biology

Figure 1 Basic properties of gumB UAS

Clp binding sites (CBSs) have typically been identified by pattern searching of the Xanthomonas campestris pv. camperstris genome using the consensus CRP binding sequence.Clp upregulates the gum operon by binding to two non-consensus sites. CBS I has a high GC content in the central region (6bp) that may be important for binding, and binding may be enhanced if the GC-rich central region is palindromic.

Usage

Figure 2 illustration of our synthetic biological system upon DSF activation

This regulatory element is used to construct our synthetic reporter construct (BBa_K3071024). It is the DNA sequence that could interact with pspF-Clp (BBa_K3071009) and lead to the activation of synthetic promoter (BBa_K3071014).

Characterization

Figure 3 rt-qPCR data Azathioprine treatment on the eforRED mRNA expression level

Azathioprine is an indirect supressor to cyclic-di-GMP, treatment of azathioprine to bacteria culture can reduce the cyclic-di-GMP level and lead to activation of the pspF-Clp and by-pass the RpfC/RpfG two-component system.The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA.

Figure 4 rt-qPCR data DSF treatment on the eforRED mRNA expression level

The result from rt-qPCR data shows that the above synthetic biological system is functional with significant up-regulation of reporter mRNA upon DSF activation. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]