DNA

Part:BBa_K3059708

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-21)


Prefix Sticky End Alpha BsaI left

Suffix BsaI sticky end alpha; exposed during Golden Gate to reveal sticky end and allow for attachment to prefix sticky end alpha. This part can be attached to any part that is compatible with 3G assembly and is compatible with CIDAR MoClo.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6


[edit]
Categories
Parameters
None