Coding

Part:BBa_K3059613:Design

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-14)


RhlR mutant


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 136
    Illegal PstI site found at 574
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 136
    Illegal PstI site found at 574
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 136
    Illegal PstI site found at 574
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 136
    Illegal PstI site found at 574
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Synthesized via Twist Bioscience.


References

1. Karig, D., Martini, K. M., Lu, T., Delateur, N. A., Goldenfeld, N., & Weiss, R. (2018). Stochastic Turing patterns in a synthetic bacterial population. Proceedings of the National Academy of Sciences, 115(26), 6572-6577. doi:10.1073/pnas.1720770115

2. Karig, D. K. (2007). Engineering multi-signal synthetic biological systems. (Doctoral dissertation). Princeton University, USA.