Coding

Part:BBa_K3059602:Design

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-14)


LasR

LasRAM is an optimized sensor from Meyer et al (2018).1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 67
    Illegal PstI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 67
    Illegal PstI site found at 475
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence from Murray Lab at California Institute of Technology.


References

1. Meyer, A. J., Segall-Shapiro, T. H., & Voigt, C. A. (2018). Marionette: E. coli containing 12 highly-optimized small molecule sensors. BioRxiv, 285866. doi:https://doi.org/10.1101/285866