Reporter

Part:BBa_K3056001:Design

Designed by: Ruben Warkentin   Group: iGEM19_Queens_Canada   (2019-09-10)


Anti-THC antibody fragment (ScFv), bound to EGFP at the C-terminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1380
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


Design Notes

EGFP was chosen over other fluorescent protein, as it is brighter than most.


Source

The antibody fragment was obtained from "The Production of Recombinant single chain Antibody Fragments for the Detection of Illicit Drug Residues", written by Dr. Brennan.

References

1. Brennan, J. (2005) The production of recombinant single chain antibody fragments for the detection of illicit drug residues. doctoral thesis, Dublin City University, [online] http://doras.dcu.ie/17319/ (Accessed March 12, 2019)